Veterinary Hematology and Clinical Chemistry,Second Edition. Veterinary,Hematology and,Clinical Chemistry,S E C O N D E DITION. Mary Anna Thrall DVM MS DACVP, Professor and Section Chief Department of Pathobiology. Ross University School of Veterinary Medicine,Basseterre St Kitts West Indies. Glade Weiser DVM DACVP, Professor Special Appointment and Clinical Pathologist. Department of Microbiology Immunology Pathology, College of Veterinary Medicine and Biomedical Sciences. Colorado State University,Fort Collins Colorado USA. Robin W Allison DVM PhD DACVP,Associate Professor Clinical Pathology. Department of Veterinary Pathobiology,Center for Veterinary Health Sciences. Oklahoma State University,Stillwater Oklahoma USA,Terry W Campbell DVM PhD. Professor Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences. Colorado State University,Fort Collins Colorado USA. A John Wiley Sons Inc Publication, This edition first published 2012 2012 by John Wiley Sons Inc. First Edition 2005 Lippincott Williams Wilkins, Wiley Blackwell is an imprint of John Wiley Sons formed by the merger of Wiley s global. Scientific Technical and Medical business with Blackwell Publishing. Editorial offices 2121 State Avenue Ames Iowa 50014 8300 USA. The Atrium Southern Gate Chichester West Sussex PO19 8SQ UK. 9600 Garsington Road Oxford OX4 2DQ UK, For details of our global editorial offices for customer services and for information about how to. apply for permission to reuse the copyright material in this book please see our website at www. wiley com wiley blackwell, Authorization to photocopy items for internal or personal use or the internal or personal use of. specific clients is granted by Blackwell Publishing provided that the base fee is paid directly to the. 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Library of Congress Cataloging in Publication Data. Veterinary hematology and clinical chemistry edited by Mary Anna Thrall. et al 2nd ed,Includes bibliographical references and index. ISBN 978 0 8138 1027 0 hardcover alk paper 1 Veterinary hematology 2 Veterinary. clinical chemistry I Thrall Mary Anna, DNLM 1 Hematologic Diseases veterinary 2 Clinical Chemistry Tests methods. 3 Clinical Laboratory Techniques veterinary 4 Hematologic Diseases diagnosis SF 769 5. SF769 5 V48 2012,636 089 60756 dc23,2012004866, A catalogue record for this book is available from the British Library. Wiley also publishes its books in a variety of electronic formats Some content that appears in print. may not be available in electronic books, Set in 9 12 pt Meridien by Toppan Best set Premedia Limited. The authors wish to dedicate this book to their mentors the pioneers in veteri. nary clinical pathology In particular the book is dedicated to Drs Maxine. Benjamin Oscar Schalm and J J Kaneko for their respective first generation. discovery and textbooks addressing veterinary clinical pathology hematology. and clinical chemistry and for their inspiration to the many subsequent careers. in veterinary clinical pathology, Dr Mary Anna Thrall wishes to thank and remember Dr Maxine Benjamin. for her generosity patience and friendship The authors acknowledge and. remember Dr E Duane Lassen for his important contributions to the first. edition of this textbook He has since lost a hard fought battle with cancer He. was an outstanding teacher excellent clinical pathologist and dear friend across. much of the veterinary clinical pathology community. Contributors ix 10 Introduction to Leukocytes and the Leukogram 118. Glade Weiser,11 Neutrophil Production Trafficking and. Preface to the First Edition xii,Kinetics 123,Glade Weiser. Section I General Principles of Laboratory 12 Interpretation of Leukocyte Responses in Disease 127. Testing and Diagnosis 1 Glade Weiser,13 Molecular Diagnostics of Hematologic. 1 Laboratory Technology for Veterinary Medicine 3,Malignancies 140. Glade Weiser,Anne Avery,2 Sample Collection Processing and Analysis of. 14 Laboratory Evaluation of Bone Marrow 150,Laboratory Service Options 34. Mary Anna Thrall and Glade Weiser,Glade Weiser,15 Lymphoproliferative Disorders and Myeloid. 3 Perspectives in Laboratory Data Interpretation and. Neoplasms 166,Disease Diagnosis 40,Mary Anna Thrall. Glade Weiser and Robin W Allison,16 Diagnosis of Disorders of Hemostasis 185. 4 Immunodiagnostics Current Use and Future Trends in. Dale C Baker,Veterinary Medicine 51, Wayne A Jensen 17 Principles of Blood Transfusion and. Crossmatching 205,Diane Brown and Linda M Vap,Section II Hematology of Common Domestic. Species 59,Section III Hematology of Common,5 Erythrocyte Morphology 61. Nondomestic Mammals Birds Reptiles,Mary Anna Thrall. Fish and Amphibians 223,6 Classification of and Diagnostic Approach. to Anemia 75 18 Mammalian Hematology Laboratory Animals and. Mary Anna Thrall Miscellaneous Species 225,Terry W Campbell. 7 Nonregenerative Anemia 81,Mary Anna Thrall 19 Hematology of Birds 238. Terry W Campbell,8 Regenerative Anemia 87,Mary Anna Thrall 20 Hematology of Reptiles 277. Terry W Campbell,9 Classification of and Diagnostic Approach to. Polycythemia 114 21 Hematology of Fish 298,Mary Anna Thrall Terry W Campbell. 22 Hematology of Amphibians 313 32 Laboratory Evaluation of the Thyroid Adrenal and. Terry W Campbell Pituitary Glands 497,Donald Meuten. 33 Parathyroid Glands and Calcium and Phosphorus,Section IV Clinical Chemistry of Common. Metabolic Pathology 545,Domestic Species 321,Donald Meuten. 23 Laboratory Evaluation and Interpretation of the. Urinary System 323,Section V Clinical Chemistry of Common. Donald Meuten,Nondomestic Mammals Birds Reptiles Fish. 24 Laboratory Evaluation of Electrolytes 378 and Amphibians 569. Andrea A Bohn, 34 Chemical Chemistry of Mammals Laboratory Animals. 25 Laboratory Evaluation of Acid Base Disorders 393. and Miscellaneous Species 571,Glade Weiser,Terry W Campbell. 26 Laboratory Evaluation of the Liver 401,35 Clinical Chemistry of Birds 582. Robin W Allison,Terry W Campbell, 27 Laboratory Evaluation of the Pancreas and Glucose. 36 Clinical Chemistry of Reptiles 599,Metabolism 425. Terry W Campbell,Robin W Allison,37 Clinical Chemistry of Fish and Amphibians 607. 28 Laboratory Evaluation of Digestion and Intestinal. Terry W Campbell,Absorption 441,Dawn Seddon, 29 Laboratory Evaluation of Plasma and Section VI Clinical Case Presentations 615. Serum Proteins 460, Robin W Allison This section includes 74 cases with clinicopathologic. data accompanied by an interpretive discussion and. 30 Laboratory Detection of Muscle Injury 476,diagnostic summary. Robin W Allison,31 Laboratory Evaluation of Lipids 480. M Judith Radin Index 750, Extra PowerPoint presentations available online at www wiley com go thrall. Contributors, Anne Avery VMD PhD Diane Brown DVM PhD DACVP M Judith Radin DVM PhD DACVP. Associate Professor and Director Assistant Professor of Pathology Professor. Clinical Immunology Laboratory Harvard Medical School Department of Veterinary Biosciences. Department of Microbiology Immunology Assistant Pathologist Department of Pathology College of Veterinary Medicine. and Pathology Laboratory Director Center for Comparative The Ohio State University. College of Veterinary Medicine and Biomedical Medicine Columbus Ohio USA. Sciences Massachusetts General Hospital, Colorado State University Boston Massachusetts USA. Dawn Seddon BVSc BVSc MSc Vet,Fort Collins Colorado USA. Path DACVP ND Microbiology,Wayne A Jensen DVM PhD MBA Clinical Pathologist. Dale C Baker DVM PhD Chief Scientific Officer Gribbles Veterinary. Diplomate ACVP ABT Morris Animal Foundation Hamilton New Zealand. Director of Pathology and Toxicology Denver Colorado USA. Portola Pharmaceuticals Inc,South San Francisco California USA. Linda M Vap DVM DACVP, Donald Meuten DVM PhD DACVP Instructor and Clinical Pathology Laboratory. Professor Coordinator, Andrea A Bohn DVM PhD DACVP Population Health and Pathobiology Department Department of Microbiology Immunology and. Associate Professor North Carolina State University Pathology. Department of Microbiology Immunology and Raleigh North Carolina USA College of Veterinary Medicine and Biomedical. Pathology Sciences, College of Veterinary Medicine and Biomedical Colorado State University. Sciences Fort Collins Colorado USA,Colorado State University. Fort Collins Colorado USA, On behalf of the contributing authors and Wiley Blackwell or complete blood count bone marrow hemostasis and. we are pleased to introduce the Second Edition of Veterinary transfusion medicine. Hematology and Clinical Chemistry Our goal is to provide an III presents hematology of common nondomestic species. image rich readable resource addressing routine laboratory encountered in veterinary practice. diagnostics in veterinary practice The theme of the presen IV presents clinical chemistry of common domestic species. tation is applied clinical pathology for veterinary students and is organized primarily by organ system. and veterinary health professional teams in the practice V presents clinical chemistry of common nondomestic. setting We aimed to maintain our intended target audience species. and original organizational structure VI is a compilation of clinical cases Each case includes a. signalment brief history and pertinent physical examina. tion findings Then relevant laboratory data are presented. Audience in tables followed by a narrative interpretation of the data. A continuing trend in frontline veterinary medicine is the. movement of laboratory diagnostics into the veterinary facil Revisions and additions. ity Evolving technological advancements in point of care. diagnostic capability drives this trend which increases the Some of the more important revisions and additions include. need for education in veterinary clinical pathology Although the following. this book was written primarily for veterinary students and The overview of laboratory technology has been updated. practitioners it has applications for a broader audience to reflect continued advances in and adoption of in clinic. serving as a useful adjunct for the educational and reference diagnostic instrumentation and capabilities Some of the his. needs of a variety of other users The following audiences torical laboratory procedures that are no longer used have. may benefit from this resource been removed Next we comment on data interpretation. students in professional veterinary medical education skills Our experience indicates that veterinarians are rea. programs sonably adept at understanding how laboratory tests relate. health professional teams in veterinary care facilities to pathophysiology but then don t think probabilistically. clinical pathologists and clinical pathologists in training about the magnitude of data abnormalities and often strug. product development groups utilizing veterinary clinical gle interpreting complex data sets Rules for interpreting. pathology diagnostic tests assume homogeneity of pathophysiologic. responses or that our animal friends have read the book. as imagined in Figure P 1, Organization However we know that there are many variables that. create considerable biologic variability in expected responses. Veterinary Hematology and Clinical Chemistry is organized into Chapter 3 Perspectives in Data Interpretation has been. six sections arranged as follows revised to provide introductory guidance to build the skill. I presents principles of laboratory technology and test set required for adroit interpretation of laboratory data This. procedures used in veterinary laboratories to generate labo involves development of flexible probabilistic thinking skills. ratory results It also presents perspectives on how labora when solving the complex puzzle formed by the array of. tory data interpretation is used in diagnosis and overall clinical findings and laboratory data. clinical case management Extensive revision and some additions have been made. II presents hematology and hemopathology of common possible for selected chapters by incorporation of content. domestic species This includes all aspects of the hemogram from new authors Examples include. Robin Allison Oklahoma State University has made, extensive revision of laboratory diagnostics related to pathol. ogy involving liver pancreas blood proteins and muscle. Andrea Bohn and Glade Weiser Colorado State Univer. sity have revised the often dreaded subjects of electrolyte. and acid base pathology with an attempt to simplify clinical. understanding of these laboratory tests, Judy Radin The Ohio State University has provided a. new chapter covering lipid pathology, Last but not least Don Meuten North Carolina State. University brings his extensive experience and expertise. cultivated from too many years in both anatomical and clini. cal pathology He contributes new treatment of renal endo. crine and calcium metabolic pathologies, The Clinical Case Presentations were a separately bound. supplement to the 1st Edition In the 2nd Edition the Clini. cal Case Presentations are incorporated into this single. bound textbook These presentations are intended to provide. students practice to develop interpretive skills by seeing. examples of how data are interpreted into pathologic pro. cesses and how pathologic processes may culminate in a. diagnostic scenario The original cases are retained because. their classical usefulness does not change In addition a. Figure P 1 Dogs caught reading the book in an attempt to make their number of new cases have been added by some of the new. disease responses predictable Not all will read the book Courtesy of contributing authors. Dr Sara Hill It is our wish that readers not only learn principles and. skills from this work but also enjoy interacting with it As. Wayne Jensen Morris Animal Foundation provides an veterinarians and specialists in bioanalytical pathology we. update of the rapidly evolving area of immunodiagnostics share our passion for the art and science of laboratory diag. Advances have continued to be made in the diagnosis and nostics applied to animal health. classification of hematopoietic cell neoplasia A new chapter Respectfully submitted. from Anne Avery Colorado State University provides an. overview of evolving molecular and flow cytometric diagnos Glade Weiser. tics applied to hematopoietic neoplasia This complements the Mary Anna Thrall. other chapters detailing leukocyte responses bone marrow Robin Allison. evaluation and hematopoietic proliferative disorders Terry Campbell. Preface to the First Edition, The publication of Veterinary Hematology and Clinical Part IV presents clinical chemistry of common domestic. Chemistry marks a new and unique contribution to veteri species and is organized primarily by organ system. nary clinical pathology The product of a collaborative effort Part V presents clinical chemistry of common nondomes. by a team of experts in the field this text combines critical tic species. information about performing diagnostic tests viewing per. tinent clinical pathology and interpreting laboratory data. with an innovative approach to incorporating color visual Unique art program. Many aspects of veterinary clinical pathology are highly. visual The most unique feature of this book is the quantity. Audience and quality of color artwork This was facilitated by digital. image acquisition and processing performed by the authors. A current trend in the field is the movement of laboratory Optimization and standardization of images was performed. diagnostics into the veterinary facility enabled by techno by digital image engineering techniques to achieve an. logical advancements in point of care diagnostic capability improvement in imagery over what is possible with conven. This movement to in house testing increases the need for tional photomicrography Our goal was to bring a new level. education in veterinary clinical pathology Although this of realism to the visual communication of concepts pertain. book was written primarily for veterinary students and prac ing to microscopy In some instances visual content has. titioners it has applications for a much broader audience been amplified by combining images from multiple micro. serving as a useful adjunct for the educational and reference scopic fields into a single figure or showing different levels. needs of a variety of other users The following audiences of magnification within the same figure Digital image engi. will benefit from this resource neering also allows for image manipulation an example is. Students in professional veterinary medical education arrangement of cells that are randomized on a microscope. programs field into a specific order to convey a concept such as cell. Veterinary health professional teams in veterinary care maturation We believe that the fidelity of visual imagery. facilities as well as its liberal integration with text content makes this. Veterinary clinical pathologists and clinical pathologists in work the first of its kind. Research and product development groups utilizing vet. erinary clinical pathology Author team, Contributing content and expertise to this project are a. Organization number of recognized authorities in the field of veterinary. clinical pathology These individuals have helped shape the. Veterinary Hematology and Clinical Chemistry is organized existing curriculum train the existing faculty and create the. into six parts arranged as follows disciplines of comparative laboratory medicine and diagnos. Part I presents principles of laboratory technology and test tic cytology as we know them today It is through the com. procedures used in veterinary labs to generate laboratory bined efforts of so many experts in the field that this book. results was made possible, Part II presents hematology and hemopathology of We hope you find this publication to be an excellent. common domestic species This includes all aspects of the resource in the clinical laboratory and for laboratory data. hemogram or complete blood count bone marrow hemo interpretation. stasis and transfusion medicine, Part III presents hematology of common nondomestic M G Weiser and M A Thrall. species encountered in veterinary practice Fort Collins Colorado.
CLINICAL PATHOLOGY Updated: January 8, 2016 _____ The Clinical Pathology Section of Veterinary Diagnostic Services provides testing in the fields of hematology, urinalysis, cytology, chemistry and endocrinology. HEMATOLOGY General information Whole blood in EDTA (purple top tube) is the required specimen for mammalian hematology.
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late 1970s-early 1980s, but in Central-Eastern Europe not until the early 1990s. In the early 21st century, women in Europe are in their late twenties when entering motherhood. Anglosaxon countries have the oldest first-time mothers, at ages around thirty, while the