Vce Vet Laboratory Skills-Books Pdf

VCE VET LABORATORY SKILLS
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2018 VET LAB SKILLS EXAM 2,SECTION A Multiple choice questions. Instructions for Section A, Answer all questions in pencil on the answer sheet provided for multiple choice questions. Choose the response that is correct or that best answers the question. A correct answer scores 1 an incorrect answer scores 0. Marks will not be deducted for incorrect answers, No marks will be given if more than one answer is completed for any question. Question 1, What information should be included on the label for an agar streak plate that has been streaked. A employee name date of birth sample identification. B employee name client name date of sample receipt. C date of sample receipt laboratory name room temperature. D employee name date of sample preparation sample identification. Question 2, When using a compound microscope with its high power objective lens which one of the following should.
not be adjusted,A coarse focus,B diaphragm,C stage clips. D fine focus,Question 3, Laboratory staff have been informed by the laboratory supervisor that a new model of sterilising equipment. will be installed in the laboratory the following week. Based on this information what action should an inexperienced technician take. A Wait for the laboratory supervisor to tell them when the training for the new equipment model will take. B Research information about the new equipment model before it arrives to help train their colleagues on. how to use it, C Research information about the new equipment model then ask the laboratory supervisor about training. opportunities, D Research information about the new equipment model and inform the laboratory supervisor that they do. not require any training,Question 4, The correct procedure is being used to prepare primary standard solutions accurately.
During this procedure quantitative transfer of a solid is used in order to. A transfer an exact mass of solute during preparation of the solution. B operate the balance according to the manufacturer s instructions. C find a probable estimate of the final concentration of the solution. D ensure the correct materials are chosen for preparation of the solution. SECTION A continued,3 2018 VET LAB SKILLS EXAM,Question 5. A dilution of potassium chloride KCl is to be prepared in the laboratory 100 mL of a 0 1 M solution of KCl. is transferred to a 200 mL volumetric flask and deionised water is added to the mark. The deionised water is best identified as the,D solution. Question 6, A laboratory technician receives a call from a client s partner requesting the client s test results. What is the most appropriate action that should be taken by the laboratory technician. A Retrieve the test results and then pass the details on to the client s partner efficiently and politely. B Request further identifying details about the client and their test and inform the partner that the client s. results are not yet ready to be released, C Request further identifying details about the client and tell the partner that they will be informed of the. test results once the results are released, D Explain that the test results cannot be released to anyone other than the client and request that the client.
calls the laboratory for the results,Question 7, Duplicate sample test values were found to be out of the expected range. When this occurs the sample test values should be,A retested every time. B ignored as an odd occurrence, C reported and noted in the workplace recording system. D treated as a serious breakdown in quality control. Question 8, Samples from a potential new supplier of hazardous chemical reagents for a laboratory. A should be tested and evaluated before use, B do not need to be supplied with safety data sheets SDS.
C should only be reordered if there are significant cost savings. D should not be used in order to avoid changes to important standard operating procedures SOP. Question 9, What is the correct ergonomic posture for a laboratory technician working with a microscope in a seated. A straight back and neck arms level with the bench knees bent 45 and toes stretched to touch the floor. B straight back and neck arms level with the bench knees bent 45 and feet resting level on the floor. C straight back and neck arms leaning forward onto the bench knees bent 45 and feet resting level on. D straight back arms leaning forward onto the bench neck stretched towards the microscope eyepieces. and legs relaxed and hanging from a high stool,SECTION A continued. 2018 VET LAB SKILLS EXAM 4,Question 10, When rotating the objective lens on a microscope to a higher powered lens the. A diaphragm must be closed,B light intensity must be increased. C light intensity must be decreased,D working distance must be increased.
Question 11, A food sample requiring urgent bacterial contamination tests has arrived at a laboratory. The most appropriate action for a laboratory technician to take would be to. A inform the laboratory supervisor about the test request and wait for the supervisor to assign the work to. a laboratory technician, B test the food sample immediately but only after referring to the test request specifications. C test the food sample immediately with a general growth media to see what grows. D check with the laboratory supervisor first before any testing is done. Question 12, The best way to sterilise an inoculating loop while minimising the formation of aerosols is to. A soak the loop in a sterilising solution for 24 hours. B place the loop directly in the blue flame of a Bunsen burner and hold it there for a full minute. C first heat the tip of the loop gradually in the flame of a Bunsen burner beginning with the handle end of. D place the loop directly in the orange flame of a Bunsen burner and pass it back and forth until the loop. Question 13, A laboratory technician prepares some agar streak plates from a broth culture During the next shift another. laboratory technician checks the agar streak plates after incubation and observes confluent growth in all. sectors of the plates, The best explanation for this observation would be that the previous laboratory technician.
A did not flame the neck of the broth bottle,B forgot to flame the loop between sectors. C was not working near the Bunsen burner flame, D forgot to clean the bench before starting the procedure for preparing the agar streak plates. Question 14, Glassware and other solid materials that require autoclaving. A do not require labelling as liquids would, B must only be sterilised with the autoclave set on a dry cycle. C must only be sterilised with the autoclave set on a wet cycle. D can be sterilised in a mixed load with the appropriate autoclave settings. SECTION A continued,5 2018 VET LAB SKILLS EXAM,Question 15.
Which one of the following would contribute the most to reducing the shelf life of a working chemical. A returning any decanted solution to the bottle, B making up a solution immediately before it is required. C keeping the solution in a refrigerator while it is not being used. D wrapping the solution in aluminium foil when it is light sensitive. Question 16, A laboratory technician would identify the physical hazard sign for an oxidising substance by which one of. the following pictograms,Sources Standard Studio Shutterstock com A C D. Migren art Shutterstock com B,Question 17, A laboratory technician has been asked to carry out a starch test on a potato sample. Which of the following pairs of stain and colour structures would show a positive result when examined. under a microscope,A Gram stain pink granules,B iodine stain brown granules.
C iodine stain blue black granules,D Gram stain purple black granules. Question 18, The laboratory supervisor in an environmental testing laboratory instructs a technician to aseptically transfer. some soil samples for microbial evaluation, Transferring aseptically in this instance would best be achieved by. A sterilising each sample by heat under steam pressure. B storing cultures in a culture collection for future study or reference. C applying a treatment that results in death or removal of all living organisms in the samples. D using procedures for handling cultures media and equipment that ensure no contamination and that. only the desired organisms are present,SECTION A continued. 2018 VET LAB SKILLS EXAM 6,Question 19, A pathology laboratory receives a notice from the National Association of Testing Authorities NATA.
Australia about an audit visit, The information that the auditors are likely to concentrate on are the. A makes and models of the laboratory equipment, B laboratory resources procedures and documentation. C profitability and efficiency of the testing procedures. D speed and efficiency with which the laboratory technicians complete their tasks. Question 20, What is the volume of a 50 stock solution that is required to prepare 200 mL of a 1 0 working solution. END OF SECTION A,7 2018 VET LAB SKILLS EXAM,SECTION B Short answer questions. Instructions for Section B,Answer all questions in the spaces provided.
Question 1 6 marks, a A pathology laboratory technician receives a call from a veterinary surgeon requesting an. urgent result on a submitted blood sample, List three pieces of information that the technician will need to obtain from the veterinary. surgeon while still on the phone 3 marks, b The veterinary surgeon has requested that the sample be tested for bacteria. What laboratory test would need to be carried out to help identify the possible strain of. bacteria 1 mark, c The test carried out in part b is expected to provide one of two possible results. Name the two possible results and give one characteristic that would help to identify each. result 2 marks,Characteristic,Characteristic,SECTION B continued.
2018 VET LAB SKILLS EXAM 8,Question 2 3 marks, A laboratory technician suspects that the electronic balance being used in the laboratory is. inaccurate, a Suggest one action that the technician could take to support the laboratory s right first time. objective 1 mark, b Identify two incidents of wastage that the inaccurate electronic balance could create within the. company if the technician did not act promptly to address the situation 2 marks. Question 3 3 marks, From the following list identify the most appropriate cleaning or disposal method for each of the. laboratory items given in the table below, biohazard waste bin sharps container 1 0 hypochlorite solution.
Laboratory item Appropriate cleaning or disposal method. glass cell counting chamber or haemocytometer,plastic flask of cell culture media. used scalpel blades from biological dissection,SECTION B continued. 9 2018 VET LAB SKILLS EXAM,Question 4 5 marks, Primary standard solutions are used extensively in analytical chemistry laboratories. a Give one reason for using primary standard solutions in laboratory work 1 mark. b What two steps should a technician take to make themselves familiar with a laboratory s. protocol for preparing primary standard solutions 2 marks. c The quantitative transfer of solids is a requirement when preparing primary standard solutions. Explain what is meant by quantitative transfer of solids and give one reason for its. importance 2 marks,SECTION B continued,2018 VET LAB SKILLS EXAM 10. Question 5 7 marks, A laboratory technician has been asked to prepare bacterial smears and to stain them using the.
Gram stain method for microscopic examination, a Before beginning observation of the slides what are two checks that should be carried out to. ensure that the microscope is fit for purpose 2 marks. b Identify the objective lens that should be used to observe the bacterial smears on the prepared. slides 1 mark, c Indicate which of the two diagrams of slides below shows the correct microscope set up for. magnification and for resolution of the image on a prepared Gram stain slide Tick the box. for your chosen slide A or B,Diagram of Slide A Diagram of Slide B. microscope lens microscope lens,immersion oil,microscope. slide immersion oil,microscope, Give two reasons for your choice of Slide A or Slide B 2 marks.
d Describe two advantages of using immersion oil on a slide 2 marks. SECTION B continued,11 2018 VET LAB SKILLS EXAM,Question 6 5 marks. a Identify two laboratory work tasks that would be included in a National Association of Testing. Authorities NATA Australia audit on quality 2 marks. b Give three reasons why quality audits should be carried out regularly 3 marks. Question 7 4 marks, The general duties of a laboratory technician include the preparation and pouring of nutrient agar. stocks for general laboratory use, What are two quality control steps a laboratory technician could take to ensure that nutrient agar. stocks are sterile Give your reasons,SECTION B continued. 2018 VET LAB SKILLS EXAM 12,Question 8 6 marks, Working in the microbiology section of a laboratory to prepare agar streak plates is one of the.
responsibilities of a laboratory technician, a Give two ways to identify contamination on agar plates that have been streaked for single. colonies with pure cultures of bacteria 2 marks, b Other than the use of personal protective equipment PPE give four approaches used to. maintain hygiene when working in the microbiology section of a laboratory 4 marks. Question 9 5 marks, A laboratory technician has been asked to prepare 250 mL of a protein solution of 2 0 w v bovine. serum albumin BSA The technician notices that the BSA protein is a very fine powder and easily. becomes airborne, a Give two actions that the technician must take in this situation to avoid possible safety. hazards 2 marks, b The standard operating procedure SOP to prepare a BSA protein solution specifies that its.
final pH must be 7 6, Identify one precaution that should be specified within the SOP for the process of dissolving. and adjusting the pH of the solution 1 mark,SECTION B Question 9 continued. 13 2018 VET LAB SKILLS EXAM, c BSA is a biological substance isolated from beef blood This blood may not have been sourced. locally but from countries that may have had outbreaks of notifiable diseases. Identify one additional health risk that this substance may present and suggest what to avoid. when handling BSA 2 marks,Health risk,What to avoid. Question 10 3 marks, Identify one hazard of working with liquid nitrogen or dry ice in a laboratory and one item of.
PPE that would need to be worn Describe the risk that may be present in the environment when. working with these substances,Item of PPE,Question 11 2 marks. The biosafety cabinet in a laboratory has been decontaminated by contractors The decontamination. procedure involves using hazardous formaldehyde vapour and isolating the laboratory where the. cabinet is located for several days However no notice of this procedure was posted at the entrance. to the laboratory in this instance, A technician has been assigned to perform a routine cell culture procedure in the laboratory the. following day and notices that the cabinet has been sealed with tape. Suggest two approaches the technician could take to deal with this situation. SECTION B continued,2018 VET LAB SKILLS EXAM 14,Question 12 5 marks. a Figure 1 below shows a blood smear from a sample. Circle the part of the blood smear that would identify separated blood cells when observing. the slide with a microscope 1 mark, b On Figure 2 below draw a line to identify and label each of the following. a white blood cell,a red blood cell,a platelet 3 marks.
c Suggest one method of checking that a blood smear stained slide will meet the quality control. standards required by a laboratory 1 mark,SECTION B continued. 15 2018 VET LAB SKILLS EXAM,Question 13 7 marks, A technician has been asked to restock a laboratory s solutions This task involves making up some. new solutions of calcium carbonate CaCO3 and potassium chloride KCl. a Complete the table below by calculating the mass required to make each solution Include the. unit of measurement and show your working 4 marks,formula weight of CaCO3 100 g mol. formula weight of KCl 74 g mol,Calculations for mass of CaCO3. Calculations for mass of KCl,Chemical Mass Molarity Volume.
CaCO3 0 4 M 350 mL,KCl 0 5 M 100 mL, b What is the most appropriate piece of equipment that should be used to store the CaCO3 and. KCl solutions 1 mark, c The technician also makes up a 70 v v ethanol solution The labelled container has the. following information 70 v v ethanol, List two other pieces of information that will need to be included on this label 2 marks.

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