Stepone And Steponeplus Real Time Pcr Systems-Books Pdf

StepOne and StepOnePlus Real Time PCR Systems
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Step Up To High Performance Real Time PCR, Remarkably Simple Systems. Applied Biosystems a leader in technology solutions for life science research introduces. its latest innovations in real time PCR the StepOne 48 well and StepOnePlus 96 well. Real Time PCR Systems These remarkably simple real time PCR systems are speci cally. designed with a user friendly yet powerful interface for both new and experienced. real time PCR researchers, Features At A Glance, StepOne System StepOnePlus System. Throughput Wells 48 96, FAM SYBR Green dyes, VIC JOE dyes. NED TAMRA dyes, Figure 1 Software Homepage VeriFlex Block. System Features, The StepOne and StepOnePlus Systems bring advanced.
real time PCR technology to a new level of accessibility even. System Highlights, for rst time users Both systems measure ampli cation as. For both novice and experienced researchers the StepOne or. it occurs cycle by cycle allowing for precise and quantitative. StepOnePlus Systems have what you need, measurements during the exponential phase of PCR Beginning. at the homepage Figure 1 you can navigate seamlessly Cost effective 3 color 48 well StepOne or 4 color 96 well. through all aspects of the real time PCR process including StepOnePlus Systems deliver precise quantitative real time. sample and reaction set up thermal cycling and uorescent PCR results. detection Focused application software analyzes and interprets Long life LED based optical system records uorescence. experimental results Depending on the experimental design. from FAM SYBR Green I VIC JOE and ROX dyes for, the system can even help you select and order real time PCR gene expression analysis pathogen quantitation SNP. reagents online by means of convenient links in the software genotyping and presence absence assays Note The. optional The system provides two fold discrimination with StepOnePlus System also accommodates the TAMRA dye. 99 7 con dence along with 9 logs of dynamic range Highly. sensitive these systems detect 10 copies of RNaseP in a 30 L. Both systems perform standard and fast PCR reactions in. reaction Additionally for laboratories with the StepOne System less than 40 minutes. that require greater throughput an upgrade kit is available Ultra compact footprint ts any laboratory setting. StepOne Upgrade LCD touchscreen and USB drive provide con guration. For those customers whose lab needs are changing Applied exibility and enable PC free operation. Biosystems offers the StepOne System upgrade Just send. Remote monitoring and email noti cation for convenience. in your existing system and you ll receive a StepOnePlus. and time savings, instrument until your upgraded system arrives in your lab. The StepOnePlus System features VeriFlex Block, Easy to Use Experimental Design Wizard.
technology which combines six independently, Both systems provide an experimental Design Wizard to. controllable peltier blocks for enhanced PCR, assist you in setting up your rst real time PCR experiment. functionality and precise temperature control, Just click on the Design Wizard and start answering questions. about the real time PCR experiment you wish to perform The The StepOne System is upgradeable to the StepOnePlus. Wizard walks you through quantitation methods detection System to meet your changing research needs. chemistry ramp speeds and nucleic acid template types. From standards to plate layout the Design Wizard guides the. entire process including reaction mix and standard dilution. calculations You re now ready to explore the simply remarkable. features of these systems, StepOnePlus System Upgrade Kit coming soon. Simply Remarkable Interface, Flexibility The StepOne and StepOnePlus Systems can be installed in.
Both the StepOne and StepOne Plus Systems adapt to multiple distinct con gurations providing unmatched exibility. almost any work ow with exible instrument control and data and convenience The unique standalone PC free con guration. management From the touchscreen control panel you can provides an ultra compact footprint that will t into any labora. quick start your experimental run without PC connectivity You tory A direct connection to a LAN enables remote monitoring. can also create a new protocol view the history of your last of experimental progress and downloading of the completed. run or see protocol details When the run is complete data run le to the PC at your desk. can be downloaded easily onto a USB ash stick or saved. System Con gurations Figure 4, to a PC Either system can be connected directly to a Local. 1 PC controlled, Area Network LAN and you can monitor the progress of the. experiment send new instructions to the system download 2 PC free. data and edit the instrument pro le The software for the two 3 Networked. systems also contains a convenient email feature that noti es. you when your experiment is complete and ready for analysis 4 PC controlled connected to LAN. 5 PC controlled with networked instrument, Figure 2 Browse Experiment Page On Touchscreen Figure 3 Run Monitor Screen On Touchscreen. Figure 4 Five Distinct Con gurations, 1 PC controlled 2 PC free 3 Networked. Software Tools for easy identi cation of sample wells when. The StepOne and StepOnePlus Systems software contains viewing ampli cation curves or SNP genotyping plots. unique features not available in other real time PCR instru. ments For example data can be analyzed from multiple. Email noti cations to alert you when a run has started. perspectives in the Multiple Plots view See Figure 5 The or ended. software constructs four plot side by side views of all data Easy cut and paste functionality. aspects including the ampli cation plot standard curve. Export easily to PowerPoint Excel or directly as a jpeg le. multi component data plots and raw data It also displays this. data next to the plate layout for easier analysis Another novel. Figure 5 StepOne System Multiple Plots View, feature is the software s ability to automatically identify wells.
that might compromise the success of an experiment During. data analysis the software generates a quality control report. table that ags wells based on criteria such as ampli cation. in a negative control well the absence of a signal in a well or. a high CT standard deviation in a replicate group This feature. reduces analysis time and allows even researchers new to. real time PCR to have con dence in their results Furthermore. you can customize this feature by disabling or modifying. quality ag settings to suit your experimental needs. Software Highlights, Experimental Design Wizards to help you design and set. up experiments, Pipetting protocols and recipes to set up. experiments quickly, Advanced set up for expert users who require exibility Figure 6 StepOne System Reaction Set up View. for more complex applications such as multiplexing. Quick start set up so you can begin a run immediately. and enter plate information later, Real time monitoring of ampli cation growth curves enables. you to view run progress can be viewed from a remote PC. Auto baseline and auto threshold for simpli ed data analysis. Multiple Plots view for simultaneous data assessment from. four perspectives, Automated SNP genotype calling with intuitive graphical.
output and quality value assignment, Troubleshooting ags to help you diagnose and solve. problematic experiments, For each target in the reaction plate the StepOne System software lets you. review and edit calculated volumes for preparing standards and PCR reactions. 4 PC controlled connected to LAN 5 PC controlled with network instrument. USB 10 100 Adapter, Simply Remarkable Applications. Instrument Chemistries Melt curve analysis, Both StepOne Systems are equipped with uorophore. Genotyping including real time ampli cation, detection chemistries that include FAM and VIC dye labeled.
TaqMan MGB probe based assays VIC and TAMRA dye, Applications. labeled probe based assays and SYBR Green I dye chemistry. The StepOne Systems support any real time PCR, Note the TAMRA dye is available only with the StepOnePlus. application Predesigned or custom assays exist for the. System TaqMan probe based assays provide outstanding. following applications, speci city and sensitivity and SYBR Green I dye chemistry is an. economical alternative for target identi cation initial screening SNP Genotyping Translocation Analysis. assays or assays that require only a few reactions Gene Expression Pro ling Gene Detection. Software Analysis MicroRNA Expression Viral Load Analysis. The software for the StepOne Systems supports a variety. of analysis methods including For information about existing Gene Expression MicroRNA and. Translocation Analysis Assays please visit www allgenes com. Absolute Quantitation, Standard curve SNP Genotyping Assay information can be found at. www allsnps com, Relative Quantitation, Relative standard curve Speed.
Comparative CT CT The StepOne and StepOnePlus Systems perform both. standard and fast thermal cycling on the same block with no. Presence absence plus minus assays with an internal modi cation Standard thermal cycling requires less than two. positive control, Figure 7 Broad Dynamic Range Figure 8 Genotyping Analysis. Ampli cation Plot Allele Discrimination Plot SNP Assay SNP Assay 1. 0 0 0 2 0 4 0 6 0 8 1 0 1 2 1 4 1 6 1 8 2 0, 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 Allele 1. Homozygous 1 1 Heterozygous 1 2 Homozygous 2 2 Undeterminded. Ampli cation plot shows log of change in normalized reporter uorescence StepOne and StepOnePlus software automatically determines genotypes and. plotted vs PCR cycle number This plot from the StepOne System illustrates 9 generates an intuitive graphic representation of results in a cluster plot report. logs of linear dynamic range for a TaqMan Assay of cDNA containing the 18S that helps you view data across populations or samples Results are from human. target sequence in tenfold serial dilutions CYP2C19 2 TaqMan SNP Genotyping Assay using the StepOnePlus System. hours and fast thermal cycling signi cantly reduces the run. times of quantitative real time PCR applications by delivering. results in 40 minutes Fast cycling is ideal for maximizing the. number of runs on an instrument in any given work day. VeriFlex Blocks, The 96 well StepOnePlus System fea. tures VeriFlex Block technology which, brings six independently controllable. peltier blocks together for precise, temperature control and enhanced.
PCR functionality VeriFlex Blocks, deliver exibility for those who have. probes and primers that are optimized, at different annealing temperatures VeriFlex Block 96 well. Because the StepOne and StepOnePlus Systems are factory. calibrated for optical and thermal accuracy simply remarkable. real time PCR results are available right out of the box Both. systems can discriminate between two populations of 5 000. and 10 000 template copies of a TaqMan assay with 99 7. con dence Both systems also demonstrate a linear dynamic. range of 9 log units or more as shown by the ampli cation. plot See Figure 7, Figure 9 Melt Curve Analysis Figure 10 Multiplex Assay. Melt Curve Multiplex Assays, Derivative Reporter Rn. 65 0 70 0 75 0 80 0 85 0 90 0 95 0 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42. Tm 79 2 Cycle, Temperature C, In this experiment standard PCR was run to generate amplicons for 18S Multiplex TaqMan Assays on the StepOnePlus System showing.
actin and GAPDH Aliquots of each amplicon were combined into one ampli cation of cDNA 96 samples using probes labeled with VIC. reaction tube and a melt curve analysis was performed and FAM reporters for 18S and TGF target sequences respectively. Systems At A Glance, dep th 51 dep th 51, 2 cm 2 0 2 cm 2 0. 16 in 16 in, height 42 7 cm 16 81 in, height 42 7 cm 16 81 in. cm 9 7 in cm 9 7 in, wid th 24 6 wid th 24 6, StepOne System StepOnePlus System. 48 wells for lower throughput 96 wells for higher throughput. 3 color for basic applications research 4 color for more exibility. Easily upgradeable to StepOnePlus System VeriFlex Block technology for thermal cycling exibility. The Remarkable, Applied Biosystems Solution, Applied Biosystems offers a complete range of real time types including animal and plant tissue cultured cells blood. PCR reagents and design analysis software to assist bacteria and yeast Blood bacteria and formalin xed material. both experienced and novice users of the StepOne and require RNA isolation kits designed speci cally for that sample. StepOnePlus Real Time PCR Systems During experimental type whereas most eukaryotic samples can be processed with. set up the Design Wizard automatically creates a material excellent results using Ambion s standard RNA isolation kits. list that links directly to the Applied Biosystems Store for. TaqMan Gene Expression Master Mix, convenient ordering of real time PCR master mixes and.
Tailored for quantitative real time PCR experiments the. consumable plasticware optional, TaqMan Gene Expression Master Mix delivers robust. Service and Support performance for both routine and challenging quantitative. Purchase of either of these systems includes a limited warranty applications It enables speci c target detection across a large. on parts and labor Depot repair and update services are dynamic range with high sensitivity down to a single copy. StepOne and StepOnePlus Real Time PCR Systems Remarkably Simple Systems Simply Remarkable Results Step Up To High Performance Real Time PCR Remarkably Simple Systems Applied Biosystems a leader in technology solutions for life science research introduces its latest innovations in real time PCR the StepOne 48 well and StepOnePlus 96 well Real Time PCR Systems These

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