Instructions For Use For Infinite 200 Pro-Books Pdf

Instructions for Use for Infinite 200 PRO
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CAREFULLY READ AND FOLLOW THE INSTRUCTIONS PROVIDED. IN THIS DOCUMENT BEFORE OPERATING THE INSTRUMENT, Every effort has been made to avoid errors in text and diagrams however Tecan. Austria GmbH assumes no responsibility for any errors which may appear in this. publication, It is the policy of Tecan Austria GmbH to improve products as new techniques. and components become available Tecan Austria GmbH therefore reserves the. right to change specifications at any time with appropriate validation verification. and approvals, We would appreciate any comments on this publication. Manufacturer, Tecan Austria GmbH, Untersbergstr 1A. A 5082 Gr dig Austria, T 43 62 46 89 33, F 43 62 46 72 770.
E mail office austria tecan com, www tecan com, Copyright Information. The contents of this document are the property of Tecan Austria GmbH and are. not to be copied reproduced or transferred to another person or persons without. prior written permission, Copyright Tecan Austria GmbH. All rights reserved, Printed in Austria, Declaration for EU Certificate. See the last page of these Instructions for Use, About the Instructions for Use. Original Instructions This document describes the Infinite 200 PRO. multifunctional microplate reader It is intended as reference and instructions for. use This document instructs how to, Install the instrument.
Operate the instrument, Clean and maintain the instrument. 2 IFU for Infinite 200 PRO No 30052730 Rev No 1 6 2016 04. Remarks on Screenshots, The version number displayed in screenshots may not always be the one of the. currently released version Screenshots are replaced only if content related to. application has changed, Trademarks, The following product names and any registered and unregistered trademarks. mentioned in this document are used for identification purposes only and remain. the exclusive property of their respective owners, TM TM TM TM. Infinite i control GCM magellan NanoQuant Plate Tecan. and the Tecan Logo are registered trademarks of, Tecan Group Ltd M nnedorf Switzerland.
Windows and Excel are registered trademarks of Microsoft Corporation. Redmond WA USA, ChromaGlo Dual Luciferase and Enliten are registered trademarks of. Promega Corporation Madison WI USA, Starna is a registered trademark of Starna Scientific Limited 52 54 Fowler. Road Hainault Essex IG6 3UT England United Kingdom. BRET2 DeepBlueC PerkinElmer AlphaScreen and AlphaLISA are. registered trademarks of PerkinElmer Inc Waltham Massachusetts USA. Warnings Cautions and Notes, The following types of notices are used in this publication to highlight important. information or to warn the user of a potentially dangerous situation. Gives helpful information, INDICATES A POSSIBILITY OF INSTRUMENT DAMAGE OR DATA LOSS. IF INSTRUCTIONS ARE NOT FOLLOWED, INDICATES THE POSSIBILITY OF SEVERE PERSONAL INJURY.
LOSS OF LIFE OR EQUIPMENT DAMAGE IF THE INSTRUCTIONS. ARE NOT FOLLOWED, THIS SYMBOL INDICATES THE POSSIBLE PRESENCE OF. BIOLOGICALLY HAZARDOUS MATERIAL PROPER LABORATORY, SAFETY PRECAUTIONS MUST BE OBSERVED. 2016 04 IFU for Infinite 200 PRO No 30052730 Rev No 1 6 3. THIS SYMBOL INDICATES THE POSSIBLE PRESENCE OF, FLAMMABLE MATERIALS AND A RISK OF FIRE PROPER. LABORATORY SAFETY PRECAUTIONS MUST BE OBSERVED, NEGATIVE ENVIRONMENTAL IMPACTS ASSOCIATED WITH THE. TREATMENT OF WASTE, DO NOT TREAT ELECTRICAL AND ELECTRONIC EQUIPMENT.
AS UNSORTED MUNICIPAL WASTE, COLLECT WASTE ELECTRICAL AND ELECTRONIC. EQUIPMENT SEPARATELY, Manufactured by, Date of manufacture. Conformit Europ enne, Read the Instructions for Use. before operating the instrument, Order number, Serial Number. WEEE symbol, RoHS Orange Logo, 4 IFU for Infinite 200 PRO No 30052730 Rev No 1 6 2016 04.
Table of Contents, 1 Safety 9, 1 1 Instrument Safety 9. 2 General Description 11, 2 1 Instrument 11, 2 1 1 Intended Use 11. 2 1 2 Multifunctionality 12, 2 1 3 Performance 13, 2 1 4 User Friendliness 13. 2 1 5 Onboard Control Button 13, 2 1 6 Rear View 14. 2 2 Software 15, 2 3 Injectors Optional 15, 2 3 1 Injector Measurement Modes 15.
2 3 2 Injector Module Diagram 16, 2 3 3 Injector Pump Options 16. 2 3 4 Storage Bottles and Bottle Holders 17, 2 3 5 Injector Carrier 18. 2 4 Measurement Techniques 20, 2 4 1 Fluorescence 20. 2 4 2 Absorbance 22, 2 4 3 Luminescence 23, 2 4 4 AlphaScreen AlphaLISA 23. 2 5 Optical System 24, 2 5 1 Fluorescence Intensity System Infinite M200 PRO 24.
2 5 2 Fluorescence Intensity System Infinite F200 PRO 30. 2 5 3 Fluorescence Polarization System Infinite F200 PRO 34. 2 5 4 Absorbance System Infinite F200 PRO 35, 2 5 5 Absorbance System Infinite M200 PRO 37. 2 5 6 Luminescence System 39, 2 5 7 Cuvette Port Infinite M200 PRO 42. 2 5 8 AlphaScreen AlphaLISA System Infinite F200 PRO only 45. 3 Installation 47, 3 1 Unpacking and Inspection 47. 3 1 1 Unpacking Procedure 48, 3 2 Removal of the Transport Locks 49. 3 3 Transport and Storage 50, 3 3 1 Transport 50, 3 3 2 Storage 50.
3 4 Power Requirements 51, 3 5 Switching the Instrument On 52. 4 Gas Control Module Enhanced 53, 2016 04 IFU for Infinite 200 PRO No 30052730 Rev No 1 6 5. 4 1 Safety 53, 4 2 Gas Control Module Features 54, 4 2 1 Gas Control Module Configurations 54. 4 2 2 Top and Rear Views of the Gas Control Module 55. 4 3 Main Menu of Modes 57, 4 3 1 CO2 Mode 57, 4 3 2 O2 Mode 58. 4 3 3 DUAL Mode 59, 4 3 4 Manual Mode 60, 4 4 Settings Menu 61.
4 5 Installing the Gas Control Module 62, 4 5 1 Requirements 62. 4 5 2 Installation Procedure 63, 4 6 Operating the Gas Control Module 67. 4 7 CO2 and N2 Gas Cylinders Not Supplied Accessory 71. 4 8 Troubleshooting the Gas Control Module 72, 5 Operating the Instrument 75. 5 1 Introduction 75, 5 2 General Operating Features 76. 5 2 1 Instrument Start Up 76, 5 3 General Options 76.
5 4 Defining Filter Slides Infinite F200 PRO only 78. 5 4 1 About Filters 78, 5 4 2 Filter Slide and Filter Orientation 78. 5 4 3 Installing a Custom Filter 80, 5 4 4 Defining the Filters 83. 5 5 Optimizing Fluorescence Measurements 87, 5 5 1 Instrument Parameters 87. 5 5 2 Z Optimization FI Top measurements with the Infinite M200 PRO only 88. 5 5 3 FI Ratio Mode 93, 5 5 4 Optimal Read 94, 5 6 FP Measurements 96. 5 6 1 Fluorescence Polarization 96, 5 6 2 Measurement Blank Range 96.
5 6 3 G Factor Settings 97, 5 6 4 Measurement with an Uncalibrated G Factor 97. 5 6 5 Measurement with a Simultaneous G Factor Calibration 98. 5 6 6 Measurement with a Calibrated G Factor 99, 5 6 7 Measurement with a Manual G Factor 100. 5 6 8 Calculation of Fluorescence Polarization Parameters 101. 5 7 Optimizing Absorbance Measurements 102, 5 7 1 Measurement Parameters 102. 6 IFU for Infinite 200 PRO No 30052730 Rev No 1 6 2016 04. 5 7 2 Absorbance Ratio Mode 102, 5 8 Multiple Reads per Well 103. 5 8 1 MRW Type 103, 5 8 2 MRW Size 104, 5 8 3 MRW Border 104.
5 8 4 Result Display in MS Excel 106, 5 8 5 Miscellaneous Software Features of MRW 106. 5 9 Optimizing Luminescence Measurements 107, 5 9 1 Integration Time 107. 5 9 2 Light Level Attenuation 107, 5 10 Optimizing AlphaScreen AlphaLISA Measurements 108. 5 10 1 Instrument Parameters 108, 5 11 Measurements with Injectors 110. 5 11 1 Priming and Washing of the Infinite 200 PRO 110. 5 11 2 Washing 114, 5 11 3 Before Starting a Measurement with Injectors 118.
5 11 4 Injector Modes i control 118, 5 12 Blanking Measurements 123. 5 13 Cuvette Measurements 124, 5 13 1 Cuvette Strip 124. 5 13 2 Cuvette Movements 124, 5 13 3 i control Cuvette Examples 125. 5 14 i control Examples 129, 5 15 Finishing a Measurement Session 133. 5 15 1 Disconnecting the Instrument 133, 5 15 2 Instrument Shut Down 133.
6 Instrument Features 135, 6 1 Introduction 135, 6 2 Instrument Specifications 136. 6 3 Fluorescence Intensity and Time Resolved Fluorescence TRF 138. 6 3 1 Definition of the Detection Limit 139, 6 3 2 Fluorescein Fluorescence Intensity Top 139. 6 3 3 Fluorescein Fluorescence Intensity Bottom 139. 6 3 4 Europium Time Resolved Fluorescence 139, 6 4 Fluorescence Polarization FP 140. 6 5 Absorbance 141, 6 6 Glow Type Luminescence 142. 6 6 1 ATP Glow Luminescence 142, 6 7 Flash Type Luminescence 143.
6 8 Dual Color Luminescence e g BRET 144, 6 9 AlphaScreen AlphaLISA 144. 6 10 On the Fly Measurements 145, 2016 04 IFU for Infinite 200 PRO No 30052730 Rev No 1 6 7. 6 11 Cuvette Features Infinite M200 PRO only 146, 6 11 1 Cuvette Specifications 146. 6 12 Injector Specifications 147, 6 12 1 Injector Reagent Compatibility 147. 6 13 Measurement Accessories 148, 6 13 1 Recommended Filters Infinite F200 PRO only 148.
6 13 2 Recommended Types of Microplates 149, 6 13 3 Luminescence Detection 153. 7 Quality Control 155, 7 1 Periodic Quality Control Tests 155. 7 2 Specifications Passed Failed Criteria 156, 7 3 Specifications Test Instructions 157. 7 3 1 Fluorescence Top 157, 7 3 2 Fluorescence Bottom 161. 7 3 3 Time Resolved Fluorescence 165, 7 3 4 Fluorescence Polarization Infinite F200 PRO only 167.
7 3 5 Glow Luminescence 169, 7 3 6 Absorbance Accuracy 170. 7 3 7 Absorbance Wavelength Accuracy 170, 7 3 8 Absorbance Baseline Flatness Infinite M200 PRO 171. 7 3 9 Absorbance Baseline Flatness Infinite F200 PRO 172. 7 3 10 Absorbance Cuvette Infinite M200 PRO only 173. 7 3 11 AlphaScreen AlphaLISA Infinite F200 PRO only 174. 8 Cleaning and Maintenance 177, 8 1 Introduction 177. 8 2 Liquid Spills 178, 8 3 Injector Cleaning and Maintenance 178. 8 3 1 Daily Maintenance 179, 8 3 2 Weekly Periodical Maintenance 179.
8 4 Instrument Disinfection 180, 8 4 1 Disinfection Solutions 180. 8 4 2 Disinfection Procedure 181, 8 4 3 Safety Certificate 181. 8 4 4 Disposal 182, 8 4 5 Disposal of Packing Material 182. 8 4 6 Disposal of Operating Material 182, 8 4 7 Disposal of the Instrument 183. 9 Troubleshooting 185, 8 IFU for Infinite 200 PRO No 30052730 Rev No 1 6 2016 04.
1 1 Instrument Safety, 1 Always follow basic safety precautions when using this product to reduce. the risk of injury fire or electrical shock, 2 Read and understand all information in the Instructions for Use Failure to. read understand and follow the instructions in this document may result. in damage to the product injury to operating personnel or poor. instrument performance, 3 Observe all WARNING and CAUTION statements in this document. 4 Never open the housing of the Infinite 200 PRO while the instrument is. plugged into a power source, 5 Never force a microplate into the instrument. 6 The Infinite 200 PRO is intended as a general purpose laboratory. instrument for professional use Observe proper laboratory safety. precautions such as wearing protective clothing and using approved. laboratory safety procedures, TECAN AUSTRIA GMBH HAS TAKEN GREAT CARE IN CREATING THE.
STORED PLATE DEFINITION FILES THAT ARE RECEIVED WITH THE. INSTRUMENT SOFTWARE, WE HAVE TAKEN EVERY PRECAUTION TO ENSURE THAT THE PLATE. HEIGHTS AND WELL DEPTHS ARE CORRECT ACCORDING TO THE DEFINED. PLATE TYPE THIS PARAMETER IS USED TO DETERMINE THE MINIMUM. DISTANCE BETWEEN THE TOP OF THE PLATE AND THE CEILING OF THE. MEASUREMENT CHAMBER ADDITIONALLY TECAN AUSTRIA HAS ADDED A. VERY SMALL SAFETY GAP TO PREVENT ANY DAMAGE OCCURRING TO THE. MEASUREMENT CHAMBER AS A RESULT OF SMALL CHANGES IN PLATE. HEIGHT THIS DOES NOT AFFECT THE PERFORMANCE OF THE. INSTRUMENT, USERS MUST ENSURE THAT THE PLATE DEFINITION FILE SELECTED. CORRESPONDS TO THE ACTUAL PLATE BEING USED, USERS SHOULD ALSO TAKE CARE THAT NO POTENTIAL FLUORESCENT. OR LUMINESCENT CONTAMINATION LIES ON TOP OF THE PLATE BE. AWARE THAT SOME PLATE SEALERS LEAVE BEHIND A STICKY RESIDUE. THAT MUST BE COMPLETELY REMOVED BEFORE STARTING, MEASUREMENTS. BEFORE STARTING MEASUREMENTS MAKE SURE THAT THE MICROPLATE. POSITION A1 IS INSERTED CORRECTLY THE POSITION OF WELL A1 HAS. TO BE ON THE UPPER LEFT SIDE, STOP TO INSURE THE OPTIMAL WORKING OF TECAN INSTRUMENTS.
WE RECOMMEND A SERVICE INTERVAL OF 6 MONTHS, 2016 04 IFU for Infinite 200 PRO No 30052730 Rev No 1 6 9. It is assumed that the instrument operators because of their vocational. experience are familiar with the necessary safety precautions for handling. chemicals and biohazardous substances, Adhere to the following laws and guidelines. 1 National industrial protection law, 2 Accident prevention regulations. 3 Safety data sheets of the reagent manufacturers, DEPENDING ON THE APPLICATIONS PARTS OF THE INFINITE 200. PRO MAY COME IN CONTACT WITH BIOHAZARDOUS INFECTIOUS. MATERIAL MAKE SURE THAT ONLY QUALIFIED PERSONNEL, OPERATE THE INSTRUMENT IN CASE OF SERVICE OR WHEN.
RELOCATING OR DISPOSING OF THE INSTRUMENT ALWAYS, DISINFECT THE INSTRUMENT ACCORDING TO THE. INSTRUCTIONS GIVEN IN THIS MANUAL, 10 IFU for Infinite 200 PRO No 30052730 Rev No 1 6 2016 04. 2 General Description, 2 General Description, 2 1 Instrument. The Tecan Infinite 200 PRO is a multifunctional microplate reader with injector. option The Infinite 200 PRO provides high performance for the vast majority of. today s microplate applications and research and is robotic compatible. 2 1 1 Intended Use, The Infinite 200 PRO has been designed as a general purpose laboratory. Every effort has been made to avoid errors in text and diagrams however Tecan Austria GmbH assumes no responsibility for any errors which may appear in this publication It is the policy of Tecan Austria GmbH to improve products as new techniques and components become available Tecan Austria GmbH therefore reserves the

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